pSpark®
Probably the best DNA cloning technology ever developed
- Blunt DNA cloning with less than 1% background
- Not a troublesome positive selection vector
- Cloning possible with 0,1 ng of non-optimized PCR
Include
20 µL pSpark® I DNA Cloning Vector 20 ng/µL, 20 µL T4 DNA Ligase 5U/Weiss, 200 µL 5X T4 DNA Ligase Buffer, 150 µL PEG 6000 10X
Brief description
pSpark® I DNA cloning kit is based on a novel breakthrough technology (patent pending) for cloning of blunt ended DNA with very high efficiency. No phosphorylated primers are needed, any proofreading polymerase may be used and vector is compatible with blue/white screening.
Contact info
Canvax Biotech SL
+34 957 348 066
info@psparkcloning.com
Files and documentation
Include
20 µL pSpark® II DNA Cloning Vector 20 ng/µL, 20 µL pSpark® TA DNA Cloning Vector 50 ng/µL, 20 µL T4 DNA Ligase 5U/Weiss, 200 µL 5X T4 DNA Ligase Buffer, 150 µL PEG 6000 10X
Brief description
pSpark® Universal DNA cloning Kit allows blunt and TA DNA cloning. Kit included a blunt DNA cloning vector called pSpark® II DNA cloning vector , based on a novel breakthrough technology (patent pending) for cloning of blunt ended DNA with very high efficiency and, pSpark® TA DNA cloning vector for TA DNA cloning. No phosphorylated primers are needed. Any DNA polymerase may be used. Both vectors are compatible with blue/white screening.
Contact info
Canvax Biotech SL
+34 957 348 066
info@psparkcloning.com
Files and documentation