pSpark®

• Over 2000 white positive colonies are routinely obtained.
• Using at least 10 times less DNA than other kits: just about 5ng per kb of insert needed for an optimal ligation

See what you have been missing: With pSpark® you can choose what you want to clone. Avoid the use of vectors that do not allow cloning of open reading frames, very short sequences, DNA sequences that behave as a promoter in E coli or a sequences that promotes frameshifting.

Efficient DNA cloning: Use pSpark® for inserts from less than 50bp to more than 10kb with a single kit (see the spotlight Cloning of long DNA fragments) instead of kits for which cloning efficiency drops sharply after 1kb.

Background of blue colonies below 1 percent of total colonies: when using cells with a transformation efficiency of 4x107 cfu/µg less than 20 blue colonies are obtained. Thus, if a high proportion of blue to white colonies appear on the plate they should be screened because probably the insert is an open reading frame, a very short sequence, a DNA sequence that behave as a promoter in E coli or a sequence that promotes frameshifting

DNA cloning is now cheaper: Avoid the use of expensive phosphorylated primers that often more than double the cost of a DNA cloning reaction.

Faster and high throughput DNA cloning: No after PCR phosphorylation step is needed. Enzymatic phosphorylation after PCR is a low efficiency step thus reducing the chance to obtain positive clones.